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If no mercury pump is available the blood gas apparatus can be used as follows The pipet IS connected with rubber tubmg to the glass tube above stopcock a and with stopcock b closed the levelmg bulb is lowered, drawing mercury from a reservoir mto the pipet.

After mixmg the blood gases are liberated by ahakmg for three minutes and the pressure ts read at a volume of 0. By taismg the levelmg bulb the liquid level is brought to the height of the stopcock capillary to expel all air from the chamber During this process no blood must enter the stopcock capillary, sinoe the octyl alcohol, floating on the surface of the blood prevents the blood from leavmg the chamber Then 0 13 ml of feme cyanide solution plus excess femeyamde to account for the stopcock bore is mtroduced Into the apparatus.

The volume of the bore is usually marked by an arrow on the filling funnel. After the stopcock has been closed and sealed with mercury the chamber is evacuated by lou'enng the mar cury to the 60 mb maik and the chamber ls shakon under vacuum for three mmutoa.

The excess of salt solution is remoyed by suction with the stopcock open to the air The mercury is lowered to the 50 mb marie and the blood-salt solution mixture is saturated with air by shaking for three mmutes.

Then the oxygen is determined os described above. The blood gases are bberated by the addition U 0 07 ml of feme cyamdeHSaponin solution plus the volume of the stopcock bore the COj is absorbed with 0 1 ml of sodium hydroxide, and the oxygen with 0.

Procedure The hemoglobm is determined simultaneously according to Sahh and by detenmnmg the total oxygen capacity If the sample b weD standardized, one division of the hemometer scale corresponds to OlOgm of hemoglobm Otherwise a correction factor must be used.

Example The sample shows a total oxygen capacity of 19 09 voL per cent. This is also the case b states of blood dilution luch as occur m nephntis with hydremia.

In a normal person the circulatmg bemoglobm is satumted with oxygen m the lungs, so that b the arterial blood about 95 por cent of the amotmt of bemoglobb corresponding to the total capacity is saturated iMth oxygen Ihe arterial oxygen saturation can bo lowered by physical labor and drop below the normal range i e 92 per cent or leas.

In nervous persons with accelerated superficial respiration a lack of oxygen m the artenal blood has been observed, correspondmg to a saturation of 90 — 80 per cent and less.

In these persons no change of air occurs b the deeper respiratory passages the partial oxygen pressure b the alveolar air ts decreased and the hemogiobta is mcomplctely saturated with oxygen.

The same is fotmd m some decompensated heart diseases; carbon monoxide poisonbg and phosgene polsoamg are some of the toxic states that lead to decreased arterial oxygen saturation.

In moderate cases of phosgene polsonmg the bek of oxygen will not be severe dunng rest, but fa considcrnblo during physical work.

The oxjgen saturation of venous blood vanes mdiflerent parte of the circulatory system and in tho antocubitol vem of a normal resting person it Is between 60 and 70 volumes per cent In patients with cyanotic heart diseases with circulatory disturbances the per centago of saturation in tho venous blood drops to 60 volume per cent or less.

Determination o? Durmg this time tho red cells wiD settle down and the sample must bo well mixed mth a glass rod before analysis. Caro must be taken to avoid all contact with oir The oxygon content of this somplo is then determined The difference betvNCcn the oxygen content of tho last and tho first sample corre- sponds to the oxygen motaboham of the erythrocytes.

Bv:m-ple Oi content of the defibnnated blood dotennmed immediately O, content of the defibnnated blood after incubation 5.

VAK SLYK0 01 4 per cent, m anemia with active rejmerative processes losses up to 70 per cent may be found This method is advantageous because it provides a quantitative measurement of the regeneration and rt mdi cates any regenerative procoes even m caaee where it can not be demonstrated histologically The results are only valid under stnctlj sterilo conditions.

In our sample tho pH according to the figures is 7. Nkill J Biel Ciem 6! If 10 ml of siKer mtrato solution use up 9.

The solution is alloivcd to cool to room temperature and is titrated with potassium thiocyanate from a nucroburette till the appearance of on orange color For the blank determination 2 ml of solution I is used and treated like tho sample.

A nse of chlondea m serum occurs during anemia, m nephntis, essential hypertensian, cardiao decompensation, unne rstentioQ hyper?

One part leadb through rubber tubmg carrying a pbch clamp to Ri the other part m the same manner to Rt The entire apparatus is held in place by a clamp attached at Ri above the ground glass piece.

The glass tubings ipaclmg to and frcm the receiver are supported by rmgs. In such a case the solution is titrated with thiosulfate till the color disappears.

Now acid is added and the titraticm perfonnod aa usual The purified NaCI still contains traces of bromine, not more than of bromme per 6 Gm, of NaCl but smee only 0 Gm.

No hypochlonte has to be added to the receiver since the chlonde content of the sample and of the fuimel washings furnish a large excess of hypochlonte.

The ondation is performed diroctly in the rccen er after the ground gloss part and the glase tubing have been nnaed carefuDj One gnun of bone acid and 0,5 Gm.

The tube la iihftLfm wnd 0. CaleulaUon A blank dctennmatlon la made whenever new reagents are pre- liarcd, the blank ordinarily ranges between 1 0 and 1 7 ml of thlcK sulfate and should bo subtracted from the volume of thiosulfate used m the analysis.

The organic substance is destroyed with chromic acid sulfunc acid m the presence of ccnc sulfate as a cata IjTjt. The flask is allowed to cool, first by itself, than by outeido coolmg, it IS then carefully diluted mth water, boDed ogam to rmove chromyl chloride, and again diluted with water to prevent tho dmclopment of SOj vnporB during distillation Now tho flask is connected to the apparatus.

Now the ansemoufl acid is added through the dropping funnel at the rate of drops per second. However these amounts will not normally occur m blood When small amounts of iodine, such as normally found m blood, are to be determmed the reduemg agent Is added until the contents of the flask turn blue-green.

This point can easily be recognised oven by an untramed observer An excess of arsemoua odd la not harmful because the lodme is libemtod instantaneously from the diction mixture.

Prolonged distillation is therefore unnecessary Clomp Hi Is now opened as far os the pressure pennits and the condenser tube becomes gradually hot all the way to the bottom of the rocciver In this way any traces of lodme left m the condenser are vaponied and can combmo with the NaOH in the receiver v ess e l s Clomp Hi IS closed m order to allow nnsing of the condcaiser tube with the receiver fluid and It is opened again to allow the fluid to return to the flask.

Ihe condenser tube is finally washed out by the condensing water vapors. Ftnallv after the elution stood for 2 mu drop of urea C to the 1 ml.

Froa 0 mL of potasaum iodide solution b then added to the taming 'up to 1 mg. After addmi tie permanganate, rotate the solution gently upon the sides d the flask and heat tho saraples for two romutes Then add 2 dn?

The iodme is liberated from the lodato by tho addition of iodide and titrated with sodrum thiosulfate. The contents must be clear and colorless, otheiTiiae a few more drops of sulfunc acid must be added The hot flask is put back on the flame and dDuto KNlnOi solution 3 IS added till the pink color remains.

S mg of phosphorus are added to 80 ml of 10 per cent tnchloracetio acid solution m a mh volumetno [lask. The solution keeps for approximately 2 weeks, when kept cold and in the dark icebox pToadurt A test tube with ground glass top is filial with 8 ml of tnchlor acetic acid and 2 mL of oxalaCed blood is added with ahaking After continued shakmg it b filtered through an ash-free filter Five mL of filtrate correspondmg to 1 ml of blood 1 mL of molybdate and 0 4 mL of ammosulfomo add reagent are pipetted into a 10 mL vdu mstno flfiak, water added up to the mark and the contenta weD muted.

At the same tune 6 ml of the working standard phosphate solutioa a or is pipetted into a 10 mL volumetne OMk, 1 mJ of molybdate and 0 4 ml of ammoaulfoDio acid reagent are added, and made up with water to the mark.

P y w colorimeter reading. The rate of dilution can be found by dotermiaing the concentratjon of the stock solution as follows mto a 60 mL measuring flask is placed 0 1 ml of SdCIj and made up to the mark with distilled water Test tubes of 2J5 cm.

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